(Solved by Humans)-BIOT600 Final exam Name: The following are one-two word answer

Question

BIOT600 Final exam???????????????????????????????????? Name:

The following are one-two word answer questions. (1pt each).

1.The bond between a phosphate group and the ribose sugar group in RNA is called which of the following? Phosphodiester linkage (bond)2.Alternative Splicing is the process that does what to the exons and introns?.3.What codon starts a protein open reading frame?? AUG4.Which of the codons cannot be recognized by a tRNA molecule? UAA, UAG, UGA5.What is the rate of DNA translation?6.During DNA replication, the 3' -OH of the growing DNA chain attacks which phosphate of an incoming nucleotide? Alpha phosphate7.The 5' capping process creates what type of linkage? 5'-5'8.Codons that code for the same amino acid are called what?9.What is the rate of mammalian DNA replication?10.What signals the end of transcription?11.What is the function of the poly A tail? Increase the stability12.What is the rate of mutation per round of DNA replication?? 10^-10 mutation per base pair per replication13.Give an example of a missense DNA mutation? Sickle-cell disease in which GAG mutated to GTG14.What provides the energy that drives the addition of nucleotides to a growing DNA chain during replication?15.Name the four RNA bases. Adenine, Uracil, Guanine, Cytosine16.What is the name of a mutation that changes the reading frame of an RNA molecule?17.How many base pairs make up a codon?18.How many amino acids are there?19.How many hydrogen bonds are formed between one A:T base pair?20.Are hairpin loops involved in the RNA splicing reaction?21.How many nucleotides specify an amino acid?22.What links codons and anti-codons together during DNA translation?23.At which position on the cytosine ring is it methylated by the methyltransferase?24.Which is the biological molecule that contains the genetic information that is transmitted hereditarily and controls the cellular functioning?25.The first procedure used for DNA sequencing and still used at times is named after its creator. Name it.26.If you inject a foreign protein into a human it will elicit what type of reaction?27.With the TALEN technology you want to recognize a 20 nucleotide sequence of DNA. How many TALEN repeats will be in the construct?28.In molecular biology what does GFP stand for?29.If you want to find information on the web about a specific miRNA what database would you search?30.The size of gene families usually increases from lower organisms to higher organisms. What is the name of one of the gene processes that has been proposed 31.due to gene homology measurements?32.Which raps around histones? RNA or DNA?33.When electron micrographs were taken of eukaryotic chromosomal DNA were hybridized with mRNA hairpins of non-base pair bonded DNA were found. What 34.structure of DNA was this the first evidence of?35.I molecular bioinformatics what does EST stand for?36.RTRACS stands for what?37.What is in the center of the Dynabeads we used in the CHiPs assay that makes it easy to pellet them.38.What is the name of the synthetic biology technique that can join multiple DNA pieces simultaneously in one reaction???39.What does IPSC stand for?

BIOT600 Final exam

Name:

The following are one-two word answer questions. (1pt each).


1.The bond between a phosphate group and the ribose sugar group in RNA is called which

of the following? Phosphodiester linkage (bond)


2.Alternative Splicing is the process that does what to the exons and introns?.



3.What codon starts a protein open reading frame? AUG



4.Which of the codons cannot be recognized by a tRNA molecule? UAA, UAG, UGA



5.What is the rate of DNA translation?



6.During DNA replication, the 3' -OH of the growing DNA chain attacks which phosphate of

an incoming nucleotide? Alpha phosphate


7.The 5' capping process creates what type of linkage? 5'-5'



8.Codons that code for the same amino acid are called what?



9.What is the rate of mammalian DNA replication?



10.What signals the end of transcription?



11.What is the function of the poly A tail? Increase the stability



12.What is the rate of mutation per round of DNA replication? 10^-10 mutation per base pair

per replication


13.Give an example of a missense DNA mutation? Sickle-cell disease in which GAG mutated

to GTG


14.What provides the energy that drives the addition of nucleotides to a growing DNA chain

during replication?


15.Name the four RNA bases. Adenine, Uracil, Guanine, Cytosine



16.What is the name of a mutation that changes the reading frame of an RNA molecule?



17.How many base pairs make up a codon?



18.How many amino acids are there?



19.How many hydrogen bonds are formed between one A:T base pair?



20.Are hairpin loops involved in the RNA splicing reaction?



21.How many nucleotides specify an amino acid?



22.What links codons and anti-codons together during DNA translation?



23.At which position on the cytosine ring is it methylated by the methyltransferase?





24.Which is the biological molecule that contains the genetic information that is transmitted

hereditarily and controls the cellular functioning?




25.The first procedure used for DNA sequencing and still used at times is named after its

creator. Name it.





26.If you inject a foreign protein into a human it will elicit what type of reaction?





27.With the TALEN technology you want to recognize a 20 nucleotide sequence of DNA.

How many TALEN repeats will be in the construct?




28.In molecular biology what does GFP stand for?





29.If you want to find information on the web about a specific miRNA what database would

you search?




30.The size of gene families usually increases from lower organisms to higher organisms.

What is the name of one of the gene processes that has been proposed 31.due to gene homology
measurements?




32.Which raps around histones? RNA or DNA?





33.When electron micrographs were taken of eukaryotic chromosomal DNA were hybridized

with mRNA hairpins of non-base pair bonded DNA were found. What 34.structure of DNA was this
the first evidence of?




35.I molecular bioinformatics what does EST stand for?





36.RTRACS stands for what?





37.What is in the center of the Dynabeads we used in the CHiPs assay that makes it easy to

pellet them.




38.What is the name of the synthetic biology technique that can join multiple DNA pieces

simultaneously in one reaction?




39.What does IPSC stand for?