(Solved by Humans)-A peptide comosed of 12 amino acids does not react with dabsyl
Question
A peptide comosed of 12 amino acids does not react with dabsyl chloride or with phenyl isothiocyanate. Cleavage with cyanogen bromide yields a peptide with a carboxyl terminal homoserine lacton residue, which is readily hydrolyzed, in turn yielding a peptide whose sequence is determined by the Edman procedure to be
E-H-F-W-D-D-G-G-A-V-L
On the other hand, cleavage with staphylococcal protease yields an equivalent of aspartate and two peptides. Use of the Edman procedure gives the following sequences for these peptides:
G-G-A-V-L-M-E and H-F-W-D
Why does the untreated peptide fail to react with dabsyl chloride or phenyl isothiocyanate?
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